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KMID : 1007520010100040341
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Food Science and Biotechnology
2001 Volume.10 No. 4 p.341 ~ p.347
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Development of Nucleic acid - Protein Complex from Tuna Spermary and the Biological Activity
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Lee, Eun Bang
Kim, Byong Kak/Shin, Jong Heon/Lee, Seung Yong/Park, Hae Ryong/Song, Ju Young/Kwon, O Gun
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Abstract
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A large amount of a nucleic acid-protein complex was extracted from tuna spermary. The extraction process and effects of this complex on cellular immune responses and anti-fatigue were investigated in this study. The nucleic acid-protein complex separated using ultrafiltration was named DW-DNA, which was composed approximately of 73% nucleic acid. When fed to ICR mice, DW-DNA increased the number of peritoneal exudate cells by two-folds compared with that in the controls. The ratio of polymorphonuclear leucocyte (PMN) cells infiltrated into the peritoneal cavity increased from 2% to 40% after oral administration of DW-DNA. As for nonspecific immune responses, the acid phosphatase production and superoxide anion secretion by activated macrophages increased by 1.6-fold and 1.3-fold, respectively, in the DW-DNA treated mice compared with the tumor controls. The anti-fatigue effect of DW-DNA was determined by testing persistency in malnourished mice on a rots-rod and swimming time in weight-loaded rats. The persistency time on the rota-rod increased by 30.7%, 12.2%, and 53% in mice fed DW-DNA at the concentrations of 50, 100, and 200 ㎎/㎏, respectively. The swimming time increased significantly (p<0.05) by 15.4%, 21.7%, and 28.7%, respectively, in mice fed DW-DNA at the same concentrations. These results indicated that the nucleic acid-protein complex can be mass-produced using ultrafiltration and may act as an immunomodulator and antifatigue agent.
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KEYWORD
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